巨噬示蹤

Fluorescent DiI Liposomes

Fluorescent DiI Liposomes: 熒光染料DiI標記的脂質體。 Fluorescent DiI Liposomes利用單核/巨噬細胞的吞噬屬性對其熒光標記，可流式細胞術（FACS）分析，共聚焦熒光顯微鏡（Confocal Microscopy ）檢測和體內的生物分布（Biodistribution）來示蹤。

DiI是一種親脂性長鏈碳菁染料，同系列還包含DiA，DiB，DiD，DiO 和 DiR。碳菁染料廣泛用作親脂性示蹤劑，用于標記細胞、細胞器、脂質體、病毒和脂蛋白。DiI在進入細胞膜之前熒光非常弱，僅當進入到細胞膜后才可以被激發出很強的熒光。

Fluorescent DiI Liposomes通過與PBS Liposomes（Blank ），Clodronate Liposomes相同的機制被單核細胞/巨噬細胞識別和吞噬。和PBS Liposomes（Blank ）對照脂質體一樣，不含氯膦酸鹽，因此單核細胞/巨噬細胞不會被清除。然而，單核細胞/巨噬細胞確實/可能通過細胞因子分泌、吞噬活性的暫時中止和文獻中描述的其他反應來對脂質體的攝入產生反應。

Highlights:

* Real-time tracking of movement, accumulation and biodistribution

* Image applications such as FACS, fluorescent microscopy and bioimaging

貨號 品名 規格 庫存 價格
CLD-8911-2ml
 Fluorescent DiI Liposomes 2ml in stock 2200.0
CLD-8911-5ml
 Fluorescent DiI Liposomes 5ml in stock 4300.0
CLD-8911-10ml
 Fluorescent DiI Liposomes 10ml in stock 7980.0
CLD-8911-15ml
 Fluorescent DiI Liposomes 15ml in stock 10900.0
CLD-8911-20ml
 Fluorescent DiI Liposomes 20ml in stock 14500.0
CLD-8911-25ml
 Fluorescent DiI Liposomes 25ml in stock 17500.0
產品詳情

品類型/Product Type:Chemicals
產品名稱/Name:Fluorescent DiI Liposomes
脂質構成/Lipid Composition:Cholesterol, L-alpha-Phosphatidylcholine
包裹藥物/Encapsulated Drug:DiI (1,1'-Dioctadecyl-3,3,3',3'- Tetramethylindocarbocyanine Perchlorate)
外觀形態/Format:Pink liquid suspensions
激發波長/Excitation:549 nm
發射波長/Emission:565nm
緩沖液/Buffer: Phosphate buffered saline (20 mM sodium phosphate, 0.9 % NaCl)
PH值/PH: 7.0-7.5
保存期/Shelf Life：3 months guaranteed for unopened vials at 2-8 oC
注意事項/Comments: Liposomes should be stored at in the dark at 4 °C and NEVER be frozen
存儲溫度/Storage: Upon receipt store at 2-8℃
運輸溫度/Shipped:Ice pack  

產品源頭

Country of origin: USA

參考文獻

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  59. Bayón LG, Izquierdo MA, Sirovich I, van Rooijen N, Beelen RH, Meijer S. Role of Kupffer cells in arresting circulating tumor cells and controlling metastatic growth in the liver. Hepatology. 1996 May;23(5):1224-31. doi: 10.1002/hep.510230542. PMID: 8621157. View article

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  63. Gaytan F, Bellido C, Morales C, García M, van Rooijen N, Aguilar E. In vivo manipulation (depletion versus activation) of testicular macrophages: central and local effects. J Endocrinol. 1996 Jul;150(1):57-65. doi: 10.1677/joe.0.1500057. PMID: 8708563. View article

  64. Karupiah G, Buller RM, Van Rooijen N, Duarte CJ, Chen J. Different roles for CD4+ and CD8+ T lymphocytes and macrophage subsets in the control of a generalized virus infection. J Virol. 1996 Dec;70(12):8301-9. doi: 10.1128/JVI.70.12.8301-8309.1996. PMID: 8970949; PMCID: PMC190917. View article

  65. de Haan A, Groen G, Prop J, van Rooijen N, Wilschut J. Mucosal immunoadjuvant activity of liposomes: role of alveolar macrophages. Immunology. 1996 Dec;89(4):488-93. doi: 10.1046/j.1365-2567.1996.d01-787.x. PMID: 9014811; PMCID: PMC1456575. View article

  66. Wolff G, Worgall S, van Rooijen N, Song WR, Harvey BG, Crystal RG. Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target organ. J Virol. 1997 Jan;71(1):624-9. doi: 10.1128/JVI.71.1.624-629.1997. PMID: 8985392; PMCID: PMC191093.. View article

  67. van Rooijen N, Sanders A. Elimination, blocking, and activation of macrophages: three of a kind? J Leukoc Biol. 1997 Dec;62(6):702-9. doi: 10.1002/jlb.62.6.702. PMID: 9400810. View article